Micron Optics - Nikon Microscope Systems for New Jersey and Puerto Rico
Eclipse Ti Inverted Series
High-speed motorized control and acquisition
The synchronized control of many motorized components such as the nosepiece, fluorescence filters, shutters, condenser turret and stage, allows researchers to use the microscope for a wide range of automated multi-dimensional experiments. Faster device movement and image acquisition decrease overall light exposure and subsequent photo-toxicity, leading to more meaningful data
Enhanced speed of individual motorized components
Operation and/or changeover speed of objectives, filter cubes, XY stage, excitation/barrier filters has been greatly enhanced, realizing stress-free operational environment that enables researchers to focus on observations and image capture routines. The newly developed controller that memorizes and reproduces observation conditions and the joystick that enables stage control at will make the microscope feel like an extension of your eyes and hands
Digital Controller Hub significantly increases motorized accessory speed
The newly developed digital Controller Hub significantly increases
motorized accessory speed by reducing the communication overhead time between
components, boosting total operation speed.
Remarkably Fast Image Acquisition
Screening image capture of 96 wells in three modes (two-channel fluorescence and phase contrast) is possible at a speed of more than twice that of conventional models.
Nikon’s exclusive and integrated Perfect Focus System (PFS) eliminates focus drift
drift is one of the biggest obstacles in time-lapse observation. Nikon’s PFS
design corrects focus drift during long-term observation and when reagents are
added. Even with high magnification, high NA objectives and techniques like
TIRF, your images are always in sharp focus. Additionally, incorporating PFS
in the nosepiece unit saves space and does not limit the use of the Ti
expanded infinity space stratum structure.
Compatible with diverse fluorescence dyes with improved performance in broader wavelength range
By now employing 870nm wavelength for the coverglass interface detection, near-infrared fluorescence dyes including Cy5.5 can be used. As the optical characteristics from ultraviolet to infrared range are also improved, the number of usable objectives is increased, realizing stable focus in applications requiring a wide range of wavelengths from Ca2+ concentration measurement in the UV to laser tweezers in the IR.
NIS-Elements Imaging Software provides secure system control
Nikon’s original imaging software NIS-Elements provides an integrated control of the microscope, cameras, components and peripherals and allows the programming of automated imaging sequences. The intuitive GUI makes setting of the experiment parameters easy and reproducible. NIS-Elements offers many tools and controls to facilitate flexible and reliable data acquisition, paired with a diverse suite of analysis tools for measurement, documentation and databasing.
6D/4D Packages Selectable Based on Application
NIS-Elements Ar (advanced research) package allows image acquisition up to 6D (X, Y, Z, time, Lambda (wavelength), multipoint) and analysis or NIS-Elements Br (basic research) which allows up to 4D image acquisition are available depending on research purposes and specimens. Upgrades are also possible by adding diverse optional modules.
For more information on NIS-Elements,
High-quality phase contrast imaging
Nikon’s world-leading optical designers have developed the unique “full intensity” external phase contrast unit. With this revolutionary system, a phase ring is incorporated in the microscope body instead of the objective lens, allowing the use of specialized objectives without phase rings and acquisition of high-quality images with high NA objectives. Moreover, using the objectives without a phase ring enables capturing of “full intensity” bright fluorescence images.
Phase Ring Incorporated in the Microscope Body
Incorporating a phase ring—that was normally positioned within the phase contrast objective lens—into the external phase contrast unit optically allows use of specified high NA objectives to produce high-resolution phase contrast images. Four types of phase contrast rings are available according to the objectives used (common for Ti-E/U/S).
Unprecedented High Resolution
Nikon’s high-performance objective lenses, including the 60x and 100x TIRF objectives with the world’s highest numerical aperture of 1.49 incorporating spherical aberration correction collars, deliver high-resolution phase contrast images that can not be captured with any standard phase contrast objective.
Bright Fluorescence Image Using Same Objective
Because there is no light loss due to a phase ring, bright “full intensity” fluorescence, confocal and TIRF images can be captured using the same objective as well as providing phase contrast observation.
Phase Contrast Observation with Water Immersion Objective
It is now possible to use a water immersion objective for phase contrast observation. Clear, high-resolution—refractive index matched—phase contrast images with minimal aberration of deep specimen areas can be captured.
High Resolution Effective for Image Analysis